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Antikörper

Aquaporin 4 Recombinant Chimeric Antibody Antibody

Produktgrößen
100μg
£689,00
HA610295-100UG
Über dieses Produkt
SKU:
HA610295
Anwendung:
IHC-Frozen, Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH06-38
Konjugat:
Non-conjugated
Immunogen:
Recombinant protein within human 224-323.
Molekulargewicht:
Predicted band size: 35 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
Western blot analysis of Aquaporin 4 on different lysates with Guinea pig anti-Aquaporin 4 antibody (HA610295) at 1/2;000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 30 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610295) at 1/2;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50;000 dilution was used for 1 hour at room temperature.