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Antikörper

Kv4.2 / KCND2 Recombinant Mouse monoclonal Antibody

Produktgrößen
100μg
£689,00
HA610272-100UG
Über dieses Produkt
SKU:
HA610272
Anwendung:
IHC-Frozen, IHC-Paraffin, Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH13-21
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 70 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Application: IHC-Fr Species: Rat Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-Kv4.2 / KCND2 antibody (HA610272) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610272) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-Kv4.2 / KCND2 antibody (HA610272) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610272) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Kv4.2 / KCND2 on different lysates with Mouse anti-Kv4.2 / KCND2 antibody (HA610272) at 1/5;000 dilution. Lane 1: Mouse cerebellum tissue lysate (no heat) Lane 2: Mouse hippocampus tissue lysate (no heat) Lane 3: Mouse brain tissue lysate (no heat) Lane 4: Mouse liver tissue lysate (negative) Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Notice: no heat means the lysate is not boiled. Lysates/proteins at 40 µg/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610272) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.