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Antikörper

BRN3A Recombinant Chimeric Antibody Antibody

Produktgrößen
100μg
£689,00
HA610252-100UG
Über dieses Produkt
SKU:
HA610252
Anwendung:
IHC-Frozen, IHC-Paraffin
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH11-65
Konjugat:
Non-conjugated
Molekulargewicht:
Predicted band size: 43 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies
Immunofluorescence analysis of frozen mouse embryo tissue with Rat anti-BRN3A antibody (HA610252) at 1/500 dilution. The section was pre-treated using 1% SDS buffer (in PBS; pH 7.4) for 5 minutes at room temperature. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature; washed with PBS; and then probed with the primary antibody (HA610252; green) at 1/500 dilution overnight at 4 ℃; washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488; HA1133) was used as the secondary antibody at 1/1;000 dilution. Nuclei were counterstained with DAPI (blue).
Immunohistochemical analysis of paraffin-embedded mouse embryonic eye tissue with Rat anti-BRN3A antibody (HA610252) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610252) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.