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Antikörper

EIF3H Recombinant Mouse monoclonal Antibody

Produktgrößen
100μg
£689,00
HA610200-100UG
Über dieses Produkt
SKU:
HA610200
Anwendung:
Western Blot, IHC-Paraffin
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH04-49
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within human EIF3H aa 1-352 / 352.
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 40 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of EIF3H on different lysates with Mouse anti-EIF3H antibody (HA610200) at 1/2;000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: MDA-MB-231 cell lysate Lane 9: MDA-MB-468 cell lysate Lane 10: MCF7 cell lysate Lane 11: RAW264.7 cell lysate Lane 12: NIH/3T3 cell lysate Lane 13: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610200) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-EIF3H antibody (HA610200) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610200) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-EIF3H antibody (HA610200) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610200) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.