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Antikörper

Annexin A3 Recombinant Mouse monoclonal Antibody

Produktgrößen
100μg
£689,00
HA610176-100UG
Über dieses Produkt
SKU:
HA610176
Anwendung:
Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
A1F7-R
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within Human Annexin A3 aa 1-323 / 323.
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 36 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Annexin A3 on different lysates with Mouse anti-Annexin A3 antibody (HA610176) at 1/2;000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: HT-29 cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: Human lung tissue lysate (40 µg/Lane) Lane 8: Mouse lung tissue lysate (40 µg/Lane) Predicted band size: 36 kDa Observed band size: 33/36 kDa Exposure time: 12 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610176) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.