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Antikörper

Human CD45 Recombinant Mouse monoclonal Antibody

Produktgrößen
100μg
£689,00
HA610158-100UG
Über dieses Produkt
SKU:
HA610158
Anwendung:
Flow Cytometry
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH03-64
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Synthetic peptide.
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 147 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Immunocytochemistry analysis of Raji cells labeling Human CD45 with Mouse anti-Human CD45 antibody (HA610158) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Human CD45 antibody (HA610158) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488; HA1125) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594; HA1122) were used as the secondary antibody at 1/1;000 dilution.
Flow cytometric analysis of human PBMC labeling Human CD45. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA610158; 1μg/mL) (red). After incubation of the primary antibody at +4℃ for 30 minutes; the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1;000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).