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Antikörper

CD10 Recombinant Mouse monoclonal Antibody

Produktgrößen
100μg
£689,00
HA610121-100UG
Über dieses Produkt
SKU:
HA610121
Anwendung:
Western Blot, IHC-Paraffin
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
A1G3-R
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Synthetic peptide within human CD10 aa 200-300.
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 86 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of CD10 on different lysates with Mouse anti-CD10 antibody (HA610121) at 1/2;000 dilution. Lane 1: Daudi cell lysate (20 µg/Lane) Lane 2: Ramos cell lysate (20 µg/Lane) Lane 3: LNCaP cell lysate (20 µg/Lane) Lane 4: HT-29 cell lysate (negative) (20 µg/Lane) Lane 5: Mouse kidney tissue lysate (40 µg/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610121) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Raji cells labeling CD10 with Mouse anti-CD10 antibody (HA610121) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD10 antibody (HA610121) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488; HA1125) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594; HA1122) were used as the secondary antibody at 1/1;000 dilution.
Immunocytochemistry analysis of Ramos cells labeling CD10 with Mouse anti-CD10 antibody (HA610121) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD10 antibody (HA610121) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488; HA1125) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594; HA1122) were used as the secondary antibody at 1/1;000 dilution.
Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Mouse anti-CD10 antibody (HA610121) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610121) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.