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Antikörper

BAX Recombinant Mouse monoclonal Antibody

Produktgrößen
100μg
£689,00
HA610038-100UG
Über dieses Produkt
SKU:
HA610038
Anwendung:
Western Blot, IHC-Paraffin
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH0-20
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Synthetic peptide within Human Bax aa 1-50 / 192.
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 21 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of BAX on different lysates with Mouse anti-BAX antibody (HA610038) at 1/5;000 dilution. Lane 1: Human kidney tissue lysate (30 µg/Lane) Lane 2: Daudi cell lysate (15 µg/Lane) Lane 3: Raji cell lysate (15 µg/Lane) Lane 4: HepG2 cell lysate (15 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 30 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610038) at 1/5;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Mouse anti-BAX antibody (HA610038) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610038) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.