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Antikörper

c-Met Recombinant Mouse monoclonal Antibody

Produktgrößen
100μg
£689,00
HA610037-100UG
Über dieses Produkt
SKU:
HA610037
Anwendung:
Western Blot, IHC-Paraffin
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
A9A4-R
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Synthetic peptide within human Met aa 1;341-1;390 / 1;390.
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 156 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of c-Met on different lysates with Mouse anti-c-Met antibody (HA610037) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 µg/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610037) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Mouse anti-c-Met antibody (HA610037) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610037) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.