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Antikörper

Human SAA1 Mouse monoclonal Antibody

Produktgrößen
100μg
£689,00
HA601333-100UG
Über dieses Produkt
SKU:
HA601333
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
A10E7
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within human SAA1 aa 19-122 (P0DJI8).
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 13.5 kDa
Physischer Zustand:
Liquid
Reinheit:
Immunogen affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Sandwich ELISA analysis of Human SAA matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA601333) diluted in carbonate/bicarbonate buffer; at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer; and incubated with serial diluted Recombinant human SAA protein (HA210927) starting from 80000 pg/ml to 0 pg/ml and detect antibody (HA601334; Biotin; 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
The concentrations of SAA were measured in duplicates; interpolated from the SAA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%.