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Antikörper

NuMA Mouse monoclonal Antibody

Produktgrößen
100μg
£689,00
HA601223-100UG
Über dieses Produkt
SKU:
HA601223
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH03-07
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within Human NuMA aa 1-200 / 2;115 (HA210958).
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 238 kDa kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Sandwich ELISA analysis of human NuMA matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA601223) diluted in carbonate/bicarbonate buffer; at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer; and incubated with serial diluted Recombinant human NuMA protein (HA210958) starting from 30000 pg/ml to 0 pg/ml and detect antibody (HA601224; Biotin; 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native NuMA in Hela extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NuMA was measured in duplicate at different sample concentrations and interpolated from the NuMA standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD; n=2). The mean NuMA concentration was determined to be 5;378 pg/mL in Hela cell extract.
Interpolated concentrations of spiked NuMA in cell culture media samples. The concentrations of NuMA were measured in duplicates; interpolated from the NuMA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD; n=2).