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Antikörper

Human IL-3 Mouse monoclonal Antibody

Produktgrößen
100μg
£689,00
HA601155-100UG
Über dieses Produkt
SKU:
HA601155
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH0-98
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant full length human IL-3 protein.
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 17.2 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Standard curve of human IL-3 matched pair antibodies: Sandwich ELISA analysis of human IL-3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH0-96] diluted in carbonate/bicarbonate buffer; at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer; and incubated with serial diluted IL-3 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH0-98]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Standard curve of human IL-3 matched pair antibodies: Sandwich ELISA analysis of human IL-3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH0-97] diluted in carbonate/bicarbonate buffer; at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer; and incubated with serial diluted IL-3 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH0-98]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.