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Antikörper

pan Cytokeratin Recombinant Mouse monoclonal Antibody

Produktgrößen
100μl
£434,00
HA600134F-100UL
Über dieses Produkt
SKU:
HA600134F
Buffer:
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol; 1% BSA; 68.98% PBS
translate.label.attr.clone:
PDH09-10
Klonalität:
Monoclonal
Konjugat:
iFluor™ 555
Host:
Mouse
Immunogen:
Full length native protein corresponding to Human pan Cytokeratin.
Isotyp:
IgG1
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Immunocytochemistry analysis of A431 cells labeling pan Cytokeratin with Mouse anti-pan Cytokeratin antibody (HA600134F) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-pan Cytokeratin antibody (HA600134F; iFluor™ 555) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.