Skip to content

Warenkorb

Der Warenkorb ist leer.

Gesamt (exkl. MwSt.) £0,00
Warenkorb überprüfen & bezahlen
Antikörper

BSA Mouse monoclonal Antibody

Produktgrößen
100μl
£400,00
HA600085-100UL
Über dieses Produkt
SKU:
HA600085
Anwendung:
ELISA, Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
A7D10
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Native protein.
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 66 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein G affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Bovine Serum Albumin Antibody (HA600085) in indirect ELISA. Indirect ELISA analysis of BSA was performed by coating wells of a 96-well plate with 100 µl per well of BSA standard diluted in carbonate/bicarbonate buffer; at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed; blocked with StartingBlock blocking buffer; and incubated with 100 µl per well of a mouse BSA monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10;000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Bovine Serum Albumin Antibody (HA600085) in indirect ELISA. Indirect ELISA analysis of BSA was performed by coating wells of a 96-well plate with 100 µl per well of BSA standard diluted in carbonate/bicarbonate buffer; at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed; blocked with StartingBlock blocking buffer; and incubated with 100 µl per well of a mouse BSA monoclonal antibody starting at a concentration of 0.8 µg/mL for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10;000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. This antibody demonstrates high specificity for BSA and little or no crossreactivity to HSA (Human serum albumin); Goat serum and Rabbit serum.
Western blot analysis of BSA on BSA protein with Mouse anti-BSA antibody (HA600085) at 1/500 dilution. Lysates/proteins at 50 µg/Lane. Predicted band size: 66 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600085) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100;000 dilution was used for 1 hour at room temperature.