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Antikörper

Calpastatin Mouse monoclonal Antibody

Produktgrößen
100μl
£400,00
HA600072-100UL
Über dieses Produkt
SKU:
HA600072
Anwendung:
Western Blot, IHC-Paraffin, Flow Cytometry
Buffer:
1*TBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
A6B1
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within human Calpastatin aa 501-708 / 708.
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 120 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein G affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600072; 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20;000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: A431 cell lysate Predicted band size: 76.5 kDa Observed band size: 120 kDa
Western blot analysis of Calpastatin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600072; 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20;000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: SiHa cell lysate Predicted band size: 76.5 kDa Observed band size: 110-120 kDa
Immunocytochemistry analysis of A549 cells labeling Calpastatin with Mouse anti-Calpastatin antibody (HA600072) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes; and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Calpastatin antibody (HA600072) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488; HA1125) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Calpastatin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA600072; 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.