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Antikörper

Actin Rabbit polyclonal Antibody

Produktgrößen
100μl
£370,00
HA500552-100UL
Über dieses Produkt
SKU:
HA500552
Anwendung:
Western Blot
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
Klonalität:
Polyclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 42 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Polyclonal Antibody
Western blot analysis of Actin on different lysates with Rabbit anti-Actin antibody (HA500552) at 1/2;000 dilution. Lane 1: Oryza sativa lysate Lane 2: Cotton lysate Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: Lane 1: 25 seconds; Lane 2: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500552) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.