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Antikörper

PCK1 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£425,00
ET7106-81-100UL
Über dieses Produkt
SKU:
ET7106-81
Anwendung:
IHC-Paraffin, Western Blot
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JU84-39
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human PCK1 aa 26-75 / 622.
Isotyp:
IgG
Molekulargewicht:
69 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Immunocytochemistry analysis of 293T cells labeling PCK1 with Rabbit anti-PCK1 antibody (ET7106-81) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃; permeabilized with 0.05% Triton X-100 in PBS for 20 minutes; and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PCK1 antibody (ET7106-81) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1;000 dilution. Nuclear DNA was labelled in blue with DAPI.
Immunocytochemistry analysis of HepG2 cells labeling PCK1 with Rabbit anti-PCK1 antibody (ET7106-81) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃; permeabilized with 0.05% Triton X-100 in PBS for 20 minutes; and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PCK1 antibody (ET7106-81) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1;000 dilution. Nuclear DNA was labelled in blue with DAPI.
Immunocytochemistry analysis of SH-SY5Y cells labeling PCK1 with Rabbit anti-PCK1 antibody (ET7106-81) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃; permeabilized with 0.05% Triton X-100 in PBS for 20 minutes; and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PCK1 antibody (ET7106-81) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1;000 dilution. Nuclear DNA was labelled in blue with DAPI.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PCK1 antibody (ET7106-81) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (ET7106-81) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.