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Antikörper

JAK1 Recombinant Rabbit monoclonal Antibody

Produktgrößen
50uL
£245,00
ET1705-84-50UL
Über dieses Produkt
SKU:
ET1705-84
Anwendung:
Western Blot, IHC-Paraffin, Flow Cytometry
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JM75-03
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human JAK1 aa 621-670 / 1;154.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 133 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of JAK1 on different lysates with Rabbit anti-JAK1 antibody (ET1705-84) at 1/1;000 dilution. Lane 1: Jurkat cell lysate Lane 2: SW480 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 133 kDa Observed band size: 133 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-84) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of JAK1 on different lysates with Rabbit anti-JAK1 antibody (ET1705-84) at 1/1;000 dilution. Lane 1: Jurkat cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: LNCaP cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 133 kDa Observed band size: 133 kDa Exposure time: 1 minute 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-84) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-JAK1 antibody (ET1705-84) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (ET1705-84) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-JAK1 antibody (ET1705-84) at 1/2;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (ET1705-84) at 1/2;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.