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Antikörper

Phospho-PLK1 (T210) Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£425,00
ET1701-33-100UL
Über dieses Produkt
SKU:
ET1701-33
Anwendung:
Western Blot, Flow Cytometry, IHC-Paraffin
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JJ20-58
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Thr210 of Human PLK1 aa 181-230 / 603.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 68 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-PLK1 (T210) on different lysates with Rabbit anti-Phospho-PLK1 (T210) antibody (ET1701-33) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Nocodazole for 24 hours cell lysate Lane 3: HeLa treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours cell lysate Lane 4: HT-29 cell lysate Lane 5: HT-29 treated with 100nM Nocodazole for 24 hours cell lysate Lane 6: HT-29 treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-33) at 1/1;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-Phospho-PLK1 (T210) antibody (ET1701-33) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (ET1701-33) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of Phospho-PLK1 (T210) was done on MCF-7 cells. The cells were fixed; permeabilized and stained with the primary antibody (ET1701-33; 1/50) (red). After incubation of the primary antibody at room temperature for an hour; the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).