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Antikörper

ATF7 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£425,00
ET1701-31-100UL
Über dieses Produkt
SKU:
ET1701-31
Anwendung:
Western Blot, IHC-Paraffin, Flow Cytometry
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JJ2-072
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human ATF7 aa 1-50 / 483.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 52 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of ATF7 on different lysates with Rabbit anti-ATF7 antibody (ET1701-31) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: RAW264.7 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time: 1 minute 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-31) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
ICC staining ATF7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde; permeabilised with 0.25% Triton X100/PBS.
ICC staining ATF7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde; permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-ATF7 antibody. Counter stained with hematoxylin.