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Antikörper

Phospho-JNK1/2/3 (T183 + T183 + T221) Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£425,00
ET1609-42-100UL
Über dieses Produkt
SKU:
ET1609-42
Anwendung:
Western Blot, IHC-Paraffin, Immunoprecipitation, Flow Cytometry, IHC-Frozen
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
ST500
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Thr183 of Human JNK1 aa 161-204 / 427.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 48/53 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-JNK1/2/3 (T183 + T183 + T221) on different lysates with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/1;000 dilution. Lane 1: Hela cell lysate; untreated (10 µg/Lane) Lane 2: Hela cell lysate; treated with Anisomycin (10 µg/Lane) Lane 3: A431 cell lysate; untreated (10 µg/Lane) Lane 4: A431 cell lysate; treated with UV40 (10 µg/Lane) Lane 5: 293 cell lysate; untreated (10 µg/Lane) Lane 6: 293 cell lysate; treated with UV40 (10 µg/Lane) Lane 7: Hela cell lysate; untreated (10 µg/Lane) Lane 8: Hela cell lysate; treated with UV40 (10 µg/Lane) Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-42) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-JNK1/2/3 (T183 + T183 + T221) on different lysates with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/2;000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 25µg/mL Anisomycin for 30 minutes cell lysate. Lysates/proteins at 20 µg/Lane. Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 2 minutes 6 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-42) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-JNK1/2/3 (T183 + T183 + T221) on different lysates with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/2;000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-42) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
ICC staining of Phospho-JNK1/2/3 (T183 + T183 + T221) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-42; 1/50) for 1 hour at room temperature; washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1;000 dilution. The nuclear counter stain is DAPI (blue).