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Antikörper

NADPH oxidase 4/NOX4 Recombinant Rabbit monoclonal Antibody

Produktgrößen
50uL
£245,00
ET1607-4-50UL
Über dieses Produkt
SKU:
ET1607-4
Anwendung:
Western Blot, IHC-Paraffin, Immunoprecipitation, Flow Cytometry
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
SY0214
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Human NOX4 aa 460-578 / 578.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 67 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of NADPH oxidase 4/NOX4 on different lysates with Rabbit anti-NADPH oxidase 4/NOX4 antibody (ET1607-4) at 1/2;000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: U-2 OS cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-4) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of NOX4 with anti-NOX4 antibody [SY0214] (ET1607-4) at 1/1;000 dilution. Lane 1: Wild-type SH-SY5Y whole cell lysate (20 µg). Lane 2/3: NOX4 fragment 1 knockdown SH-SY5Y whole cell lysate (20 µg). Lane 4/5: NOX4 fragment 2 knockdown SH-SY5Y whole cell lysate (20 µg). Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1607-4; 1/1;000) and Loading control antibody(Rabbit anti-HSP90; ET1605-56; 1/10;000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1/200;000 dilution was used for 1 hour at room temperature.
NADPH oxidase 4/NOX4 was immunoprecipitated in 0.2mg SH-SY5Y cell lysate with ET1607-4 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1607-4 at 1/1;000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5;000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y cell lysate (input) Lane 2: Rabbit IgG instead of ET1607-4 in SH-SY5Y cell lysate Lane 3: ET1607-4 IP in SH-SY5Y cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL: K1801
Immunocytochemistry analysis of SH-SY5Y cells labeling NADPH oxidase 4/NOX4 with Rabbit anti-NADPH oxidase 4/NOX4 antibody (ET1607-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NADPH oxidase 4/NOX4 antibody (ET1607-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.