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Antikörper

AP-4 complex subunit sigma Rabbit polyclonal Antibody

Produktgrößen
100μl
£370,00
ER1902-15-100UL
Über dieses Produkt
SKU:
ER1902-15
Anwendung:
IHC-Paraffin, ELISA
Buffer:
1*PBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
Klonalität:
Polyclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within thaliana AP-4 complex subunit sigma aa 51-100 / 143.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 17 kDa
Physischer Zustand:
Liquid
Reinheit:
Immunogen affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Polyclonal Antibody
Immunofluorescence staining of paraffin- embedded A. thaliana using anti-AP-4 complex subunit sigma rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature; washed with PBS; and then probed with ER1902-15 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-AP-4 complex subunit sigma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (ER1902-15; 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.