Skip to content

Warenkorb

Der Warenkorb ist leer.

Gesamt (exkl. MwSt.) £0,00
Warenkorb überprüfen & bezahlen
Antikörper

CD42b Mouse monoclonal Antibody

Produktgrößen
100μl
£400,00
EM1902-15-100UL
Über dieses Produkt
SKU:
EM1902-15
Anwendung:
IHC-Paraffin, Flow Cytometry
Buffer:
1*PBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
13G2
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within human CD42b aa 100-350 / 652.
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 72 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD42b antibody (EM1902-15) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (EM1902-15) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Mouse anti-CD42b antibody (EM1902-15) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (EM1902-15) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of CD42b was done on Jurkat cells. The cells were fixed; permeabilized and stained with the primary antibody (EM1902-15; 1/50) (red). After incubation of the primary antibody at room temperature for an hour; the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).