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Antikörper

Albumin Mouse monoclonal Antibody

Produktgrößen
100μl
£400,00
EM1901-81-100UL
Über dieses Produkt
SKU:
EM1901-81
Anwendung:
Western Blot, Flow Cytometry, ELISA
Buffer:
1*PBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
1-G10
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Native protein.
Isotyp:
IgG1
Molekulargewicht:
69 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Albumin on human plasma sample. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-81; 1/50000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5;000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of Albumin was done on HepG2 cells. The cells were fixed; permeabilized and stained with the primary antibody (EM1901-81; 1/100) (red). After incubation of the primary antibody at room temperature for an hour; the cells were stained with Alexa FluorTM488 Goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Albumin Antibody (EM1901-81) in indirect ELISA. Indirect ELISA analysis of Albumin was performed by coating wells of a 96-well plate with 50 µl per well of human albumin antigen or BSA diluted in carbonate/bicarbonate buffer; at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed; blocked with StartingBlock blocking buffer; and incubated with 50 µl per well of a mouse Albumin monoclonal antibody starting at a concentration of 2 µg/mL and serially diluting it to a concentration of 1.95 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10;000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.