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Antikörper

Antithrombin III / ATIII / SERPINC1 Mouse monoclonal Antibody

Produktgrößen
100μl
£400,00
EM1901-11-100UL
Über dieses Produkt
SKU:
EM1901-11
Anwendung:
Western Blot, IHC-Paraffin, Flow Cytometry
Buffer:
1*PBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
15F1
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within Human SERPINC1 aa 150-370 / 464.
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 53 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Antithrombin III / ATIII / SERPINC1 on different lysates with Mouse anti-Antithrombin III / ATIII / SERPINC1 antibody (EM1901-11) at 1/1;000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse brain tissue lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 23 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-11) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Antithrombin III / ATIII / SERPINC1 on HL-60 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-11; 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Antithrombin III / ATIII / SERPINC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-11; 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5;000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: SiHa cell lysate Lane 3: U937 cell lysate
Western blot analysis of Antithrombin III / ATIII / SERPINC1 on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-11; 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5;000 dilution was used for 1 hour at room temperature.