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Antikörper

Carbonic anhydrase 2 Mouse monoclonal Antibody

Produktgrößen
100μl
£400,00
EM1801-08-100UL
Über dieses Produkt
SKU:
EM1801-08
Anwendung:
Western Blot, IHC-Paraffin
Buffer:
1*PBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
11A1
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within Human Carbonic anhydrase 2 aa 63-240 / 260.
Isotyp:
IgG2a
Molekulargewicht:
Predicted band size: 29 kDa
Physischer Zustand:
Liquid
Reinheit:
Immunogen affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Carbonic anhydrase 2 on different cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1/1;000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5;000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate; untreated Lane 2: HL-60 cell lysate; untreated
Western blot analysis of Carbonic anhydrase 2 on different lysates with Mouse anti-Carbonic anhydrase 2 antibody (EM1801-08) at 1/500 dilution. Lane 1: A431 cell lysate Lane 2: 293 cell lysate Lane 3: Human kidney tissue lysate(20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-08) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20;000 dilution was used for 1 hour at room temperature.
ICC staining Carbonic anhydrase 2 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Carbonic anhydrase 2 monoclonal antibody at a dilution of 1/100 for at least 1 hour at room temperature; washed with PBS. Alexa Fluor™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ICC staining Carbonic anhydrase 2 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Carbonic anhydrase 2 monoclonal antibody at a dilution of 1/100 for at least 1 hour at room temperature; washed with PBS. Alexa Fluor™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).