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Antikörper

GST Rabbit polyclonal Antibody

Produktgrößen
100μl
£370,00
1003-8-100UL
Über dieses Produkt
SKU:
1003-8
Anwendung:
Western Blot, ELISA, Immunoprecipitation
Buffer:
1*PBS (pH7.4); 0.2% BSA; 25% Glycerol. Preservative: 0.05% Sodium Azide.
Klonalität:
Polyclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within GST protein.
Isotyp:
IgG
Physischer Zustand:
Liquid
Reinheit:
Immunogen affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Polyclonal Antibody
  • Datenblatt des Herstellers:1003-8
GST was immunoprecipitated in 0.2mg 293T-OE-GST cell lysate with 1003-8 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using 1003-8 at 1/5;000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5;000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with GST-tagged Histone H3.1 cell lysate (input) Lane 2: Rabbit IgG instead of 1003-8 in 293T transfected with GST-tagged Histone H3.1 cell lysate Lane 3: 1003-8 IP in 293T transfected with GST-tagged Histone H3.1 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds
Western blot analysis on GST fusion protein (26kDa) using anti-GST polyclonal antibody.
Immunocytochemistry analysis of 293T cells transfected with or without GST labeling GST with Rabbit anti-GST antibody (1003-8) at 1/2;000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GST antibody (1003-8) at 1/2;000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.