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Antikörper

Histone H2A.X (phospho Ser139) rabbit pAb

Produktgrößen
50UL
£ POA
ES1326-50UL
100UL
£ POA
ES1326-100UL
Über dieses Produkt
SKU:
ES1326
Zusätzliche Namen:
H2AFX; H2AX; Histone H2A.x; H2a/x
Anwendung:
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Klonalität:
Polyclonal
Konzentration:
1 mg/ml
Weitere Details:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A; H2B; H3; and H4) form an octamer; around which approximately 146 bp of DNA is wrapped in repeating units; called nucleosomes. The linker histone; H1; interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family; and generates two transcripts through the use of the conserved stem-loop termination motif; and the polyA addition motif. [provided by RefSeq; Oct 2015];
Immunogen:
The antiserum was produced against synthesized peptide derived from human Histone H2A.X around the phosphorylation site of Ser139. AA range:94-143
Isotyp:
IgG
Reaktivitäten:
Human, Mouse, Rat, Hamster
Versandbedingungen:
Blue Ice
Quelle:
Rabbit
Lagerbedingungen:
-20[o]C
Hersteller:
ELK Biotechnology
Typ:
Antibodies: Polyclonal Antibody
Western blot analysis of lysates from 1) 4T1; 2) 293 cells; (Green) primary antibody was diluted at 1:1000; 4°over night; Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000; 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (Immunoway:YM3028) antibody was diluted at 1:5000 as loading control; 4° over night;Dylight 680 secondary antibody(Immunoway:RS23710)was diluted at 1:10000; 37° 1hour.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1;Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200(4°C;overnight). 2; Sodium citrate pH 6.0 was used for antibody retrieval(>98°C;20min). 3;Secondary antibody was diluted at 1:200(room tempeRature; 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1;Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200(4°C;overnight). 2; Sodium citrate pH 6.0 was used for antibody retrieval(>98°C;20min). 3;Secondary antibody was diluted at 1:200(room tempeRature; 30min). Negative control was used by secondary antibody only.
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