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Assay-Kits & ELISAs

Human C5aR1(Complement Component 5a Receptor 1) ELISA Kit

Produktgrößen
96T
£ POA
ELK9684-96T
48T
£ POA
ELK9684-48T
96T*5
£ POA
ELK9684-96TX5
Über dieses Produkt
SKU:
ELK9684
Weitere Details:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C5aR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C5aR1. Next; Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added; only those wells that contain Human C5aR1; biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C5aR1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
4[o]C/-20[o]C
Hersteller:
ELK Biotechnology
Typ:
Assay Kits: ELISAs
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