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Assay-Kits & ELISAs

Cattle TLR4(Toll Like Receptor 4) ELISA Kit

Produktgrößen
96T
£ POA
ELK5795-96T
48T
£ POA
ELK5795-48T
96T*5
£ POA
ELK5795-96TX5
Über dieses Produkt
SKU:
ELK5795
Weitere Details:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle TLR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TLR4. Next; Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added; only those wells that contain Cattle TLR4; biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TLR4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
4[o]C/-20[o]C
Hersteller:
ELK Biotechnology
Typ:
Assay Kits: ELISAs
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