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Assay-Kits & ELISAs

Human PEDF(Pigment Epithelium Derived Factor) ELISA Kit

Produktgrößen
96T
£ POA
ELK2723-96T
48T
£ POA
ELK2723-48T
96T*5
£ POA
ELK2723-96TX5
Über dieses Produkt
SKU:
ELK2723
Weitere Details:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PEDF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PEDF. Next; Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added; only those wells that contain Human PEDF; biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PEDF in the samples is then determined by comparing the OD of the samples to the standard curve.
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
4[o]C/-20[o]C
Hersteller:
ELK Biotechnology
Typ:
Assay Kits: ELISAs
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