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Assay-Kits & ELISAs

Mouse IL10(Interleukin 10) ELISA Kit

Produktgrößen
96T
ELK1143-96T
96T*5
ELK1143-96T*5
48T
ELK1143-48T
Über dieses Produkt
SKU:
ELK1143
Weitere Details:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL10. Next; Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added; only those wells that contain Mouse IL10; biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL10 in the samples is then determined by comparing the OD of the samples to the standard curve.
Reaktivitäten:
Mouse
Versandbedingungen:
Blue Ice
Lagerbedingungen:
4[o]C/-20[o]C
Hersteller:
ELK Biotechnology
Typ:
Assay Kits: ELISAs
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