Mini Sample Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
Produktgrößen
24 tests
£143,00
E-MSEL-R0001-24TESTS
48 tests
£302,00
E-MSEL-R0001-48TESTS
96 tests
£365,00
E-MSEL-R0001-96TESTS
Über dieses Produkt
- SKU:
- E-MSEL-R0001
- Zusätzliche Namen:
- TIMP1,CLGI,EPA,EPO,HCI
- Anwendung:
- ELISA
- Weitere Details:
- Matrix metalloproteinases (MMPs), also called matrixins, constitute a family of zinc and calciumdependent endopeptidases that function in the breakdown of extracellular matrix and in theprocessing of a variety of biological molecules. They play an important role in many normalphysiological processes such as embryonic development, morphogenesis, reproduction andtissue remodeling (1). They also participate in many pathological processes such as arthritis,cancer and cardiovascular disease (2). While the amounts of newly synthesized MMPs areregulated mainly at the levels of transcription, the proteolytic activities of existing MMPs arecontrolled through both the activation of proenzymes or zymogens and the inhibition ofactive enzymes by endogenous inhibitors such as A Alpha2-macroglobulins and tissue inhibitors ofmetalloproteinases (TIMPs).The mammalian TIMP family includes four members that share structural similarity (3). All TIMPproteins have 12 conserved cysteine residues that form six intrachain disulfide bonds, resultingin an extremely stable protein with six loops. The TIMP protein has two structurally andfunctionally distinct domains: the N-terminal domain consisting of loops 1-3 that areresponsible for tight, but non-covalent binding to the active MMPs in a 1:1 stoichiometry, andthe C-terminal domain consisting of loops 4-6 that enhances the enzyme-inhibitor interactions.In the case of TIMP-1, the C-terminal domain has also been shown to bind the hemopexin-likedomain of pro-MMP-9. TIMP-1 stimulates erythropoiesis, inhibits angiogenesis and is an antiapoptotic agent for B cells. These TIMP-1 functions may be independent of MMP inhibition(4-6).Rat TIMP-1 is a 28-35 kDa secreted glycoprotein (6, 7). The protein is synthesized as a 217 aminoacid (aa) precursor that contains a 23 aa signal peptide and a 194 aa mature form (8). RatTIMP-1 shares 85%, 70%, 68%, and 66% aa sequence identity with its mouse, human, porcine,and canine counterparts, respectively (9-13). Among the three known rat TIMPs, TIMP-1 shares40% and 35% aa identity with TIMP-2 and TIMP-3, respectively (14, 15). TIMP-1 is widelyexpressed in many cells such as fibroblasts, osteoblasts, endothelial cells, granulosa cells,dendritic cells, vascular smooth muscle cells, adipocytes, and monocytes (6, 16-19).The Quantikine® Rat TIMP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measurerat TIMP-1 levels in cell culture supernates, serum, and plasma. It contains NS0-expressedrecombinant rat TIMP-1 and antibodies raised against the recombinant protein. Natural ratTIMP-1 showed dose-response curves that were parallel to the standard curves obtained usingthe Quantikine® kit standards. These results indicate that this kit can be used to determine .relative levels of natural rat TIMP-1.
- Format:
- Colorimetric method,ELISA,Sandwich
- Physischer Zustand:
- Other Biological Fluid, Plasma, Serum
- Reichweite:
- 0.31-20 ng/mL
- Reaktivitäten:
- Rat
- Empfindlichkeit:
- 0.19 ng/mL
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Rat
- Lagerbedingungen:
- 2-8[o]C
- Hersteller:
- Elabscience
- Typ:
- Assay Kits: ELISAs
- Datenblatt des Herstellers:p-mini_sample_rat_timp_1_(tissue_inhibitors_of_metalloproteinase_1)_elisa_kit-e_msel_r0001