NAD+/NADH Colorimetric Assay Kit (WST-8)
Produktgrößen
96 tests
£397,00
E-BC-K804-M-96TESTS
Über dieses Produkt
- SKU:
- E-BC-K804-M
- Zusätzliche Namen:
- NAD+/NADH
- Weitere Details:
- Detect total content of NAD+ and NADH: Ethanol generates acetaldehyde under the action of enzyme. Meanwhile, NAD+ is reduced to NADH, NADH, under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NAD+ and NADH can be quantified by measure the OD value at 450 nm. Detect NADH: After treating sample, heat at 60oC water bath for 30 min. the NAD+ of the sample is decomposed and only NADH remains. NADH reduces WST-8 to form yellow product, and the amount of NADH is determined by measure the OD value at 450 nm. Detect NAD+ and NAD+/NADH: The content of NAD+ and the ratio of NAD+/NADH in the sample can be obtained according to the total content of NAD+ and NADH obtained of the first two steps as well as the separate content of NADPH. Note: NADP+ and NADPH have no effect on the determination results.
- Format:
- Colorimetric
- Physischer Zustand:
- Tissue
- Reichweite:
- 0.02-5.0 umol/L
- Empfindlichkeit:
- 0.02 umol/L
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- -20[o]C Avoid freeze/thaw cycles. Protect from light.
- Hersteller:
- Elabscience
- Typ:
- Assay Kits: Detection
- Datenblatt des Herstellers:nadh_colorimetric_assay_kit_(wst_8)-e_bc_k804_m