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Assay-Kits & ELISAs

Sirtuin 1 (SIRT-1) Activity Assay Kit

Produktgrößen
48 tests
£340,00
E-BC-F056-48TESTS
96 tests
£451,00
E-BC-F056-96TESTS
Über dieses Produkt
SKU:
E-BC-F056
Weitere Details:
The Sirtuins (SIRT) family of proteins includes SIRT-1 and SIRT-7, which are histone deacetylases with nicotinamide adenine dinucleotide (NAD+) dependence and are commonly expressed in organs and tissues. Based on the amino acid sequence, the Sirtuins family proteins can be divided into four types: SIRT-1 and SIRT-3 are type 1, SIRT-4 is type 2, SIRT-5 is type 3, and SIRT-6 and SIRT-7 are type 4. SIRT-1 is widely distributed in organs and tissues, mainly present in the cytoplasm, and needs to enter the nucleus to function. Some drugs can regulate the expression of certain genes by inhibiting SIRT-1 entry into the nucleus. This kit uses the fluorescence resonance energy transfer (FRET) method for detection. After deacetylation by SIRT-1, the fluorescent substrate generates an activated fluorescent substrate, which can be decomposed by proteases to produce fluorescent substances. The maximum excitation wavelength of fluorescent substances is 340 nm, and the maximum emission wavelength is 440 nm.
Format:
Fluorometric method
Immunogen:
SIRT-1
Physischer Zustand:
Tissue
Reichweite:
0.03-9.66 U/L
Empfindlichkeit:
0.03 U/L
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles. Protect from light.
Hersteller:
Elabscience
Typ:
Assay Kits: Detection