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Antikörper

Cleaved Caspase-9 P37 Polyclonal Antibody

Produktgrößen
60 ul
£235,00
E-AB-93363-60UL
120 ul
£346,00
E-AB-93363-120UL
200 ul
£541,00
E-AB-93363-200UL
Über dieses Produkt
SKU:
E-AB-93363
Zusätzliche Namen:
APAF-3;APAF3;CASP9;Caspase 9;ICE-LAP6;MCH6;PPP1R56;casp9;caspase-9
Anwendung:
Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS
Klonalität:
Polyclonal
Konzentration:
1 mg/ml
Weitere Details:
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Host:
Rabbit
Immunogen:
Recombinant fusion protein of human Cleaved Caspase-9 P37
Isotyp:
IgG
Aufreinigung:
Affinity Purified
Reaktivitäten:
Human, Mouse, Rat
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Polyclonal Antibody
Western blot analysis of extracts of Jurkat using Cleaved Caspase-9 P37 Polyclonal Antibody at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37°C for 5 hours.
Immunohistochemistry of paraffin-embedded human liver cancer using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat kidney using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HepG2 cells using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.