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Antikörper

Recombinant CD16-2/FCGR4 Monoclonal Antibody

Produktgrößen
100 ul
£402,00
AN300462P-100UL
Über dieses Produkt
SKU:
AN300462P
Zusätzliche Namen:
Fcgr,Fcgr4,4833442P21Rik,4833442P21Rik Protein,CD16-2,CD16-2 Protein,FcgammaRIV,FcgammaRIV Protein,Fcgr3a,Fcgr3a Protein,FcgRIV,FcgRIV Protein,Fcrl3,Fcrl3 Protein,Low Affinity Immunoglobulin Gamma Fc Region Receptor IV,Mouse
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Buffer:
PBS
translate.label.attr.clone:
B388
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
FcgRIV, also known as CD16-2, is an intermediate-affinity activating receptor for IgG2a and IgG2b. CD16-2 is the mouse homolog of human FcgRIIIA. CD16-2 is a low-affinity IgE receptor for all allotypes and the ligation of FcgRIV by antigen-IgE immune complexes promotes macrophage-mediated phagocytosis and is involved in lung inflammation.
Host:
Rabbit
Immunogen:
Recombinant Mouse FCGR4 protein
Isotyp:
IgG
Aufreinigung:
Protein A Purified
Reaktivitäten:
Mouse
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of Mouse FcGR4 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse FcGR4 monoclonal antibody (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow cytometric analysis of FCGR4 expression on Raw264.7 cells. Mouse leukaemic monocyte macrophage cell line RAW 264.7 were stained with anti-mouse FCGR4, then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.