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Antikörper

Recombinant ERP72/PDIA4 Monoclonal Antibody

Produktgrößen
100 ul
£402,00
AN300439P-100UL
Über dieses Produkt
SKU:
AN300439P
Zusätzliche Namen:
PDIA,Protein Disulfide-Isomerase A,ERP,PDIA4,ERP70,ERP72,ERp-72,Endoplasmic Reticulum Resident Protein 70,Endoplasmic Reticulum Resident Protein 72,ER Protein 70,ER Protein 72,Protein Disulfide-Isomerase A4,Calcium binding protein intestinal related,EPR70,EPR72,ERP 70,ERp 72,intestinal-related),PDIA 4,PDIR,Protein disulfide isomerase A4,Protein disulfide isomerase associated 4,Protein disulfide isomerase family A member 4,Protein disulfide isomerase related protein,protein disulfide isomerase related protein (calcium-binding protein,Protein ERp 72,Protein ERp72
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS
translate.label.attr.clone:
B365
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly.
Host:
Rabbit
Immunogen:
Recombinant Human ERP72/PDIA4 Protein
Isotyp:
IgG
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Immunoprecipitation analysis using 2 uL anti-PDIA4 Monoclonal Antibody and 60 ug of Immunomagnetic beads Protein G. Western blot was performed from the immunoprecipitate using PDIA4 Monoclonal Antibody at a dilution of 1:100. Lane A:0.5 mg Hela Whole Cell Lysate, Lane B:0.5 mg MCF-7 Whole Cell Lysate
Western Blot with PDIA4 Monoclonal Antibody at dilution of 1:500 dilution. Lane A: A431 Whole Cell Lysate, Lane B: NIH-3T3 Whole Cell Lysate, Lane C: 293T Whole Cell Lysate, Lysates/proteins at 30 ug per lane.
Flow cytometric analysis of Human PDIA4 expression on HepG2 cells. The cells were treated according to manufacturer's manual, stained with purified anti-Human PDIA4, t
Immunofluorescence analysis of PDIA4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PDIA4 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.