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Antikörper

Recombinant Phospho-Histone H2A.X (Ser139) Monoclonal Antibody

Produktgrößen
20 ul
£167,00
AN300358L-20UL
100 ul
£402,00
AN300358L-100UL
Über dieses Produkt
SKU:
AN300358L
Zusätzliche Namen:
H2A.X,H2A/X,H2AX,Histone H2AX,H2AFX,gamma H2A.X,G GammaH2AX
Anwendung:
IHC-Paraffin, Western Blot
Buffer:
10 mM sodium HEPES, 150 mM NaCl, 100 ug/mL protein protectant, 50% glycerol, pH 7.5
translate.label.attr.clone:
B280
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Host:
Rabbit
Immunogen:
A synthetic peptide corresponding to residues around Ser139 of Human Phospho-Histone H2A.X
Isotyp:
IgG
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer tissue using Histone H2A.X (Ser139) Monoclonal Antibody at dilution of 1:200.
Western blot analysis of extracts from serum-starved NIH-3T3, untreated(line A) or treated with UV (30min; +)(line B), using Phospho-Histone H2A.X (Ser139) rabbit monoclonal Antibody at 1:5000 dilution. (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution(lower).
Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with UV (30min; +) (line B); treated with UV and L Lambda-phosphatase (line C) using Phospho-Histone H2A.X (Ser139) rabbit monoclonal Antibody at 1:5000 dilution.
Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with UV (30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Histone H2A.X (Ser139) rabbit monoclonal Antibody at 1:5000 dilution.