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Antikörper

Recombinant PSPH Monoclonal Antibody

Produktgrößen
100 ul
£402,00
AN300269P-100UL
Über dieses Produkt
SKU:
AN300269P
Zusätzliche Namen:
PSPH,PSP,PSPHD,L-3-Phosphoserine Phosphatase,O-Phosphoserine Phosphohydrolase,Phosphoserine Phosphatase,PSPase
Anwendung:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS
translate.label.attr.clone:
B187
Klonalität:
Recombinant Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome.
Host:
Rabbit
Immunogen:
Recombinant Human PSPH protein
Isotyp:
IgG
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Recombinant Antibodies
Immunofluorescence analysis of Human PSPH in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PSPH Monoclonal Antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI for nuclear staining (blue).
Immunoprecipitation analysis using 0.5 uL anti-PSPH Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using PSPH Monoclonal Antibody at a dilution of 1:1000. Lane A:0.5 mg K562 Whole Cell Lysate, Lane B:0.5 mg HepG2 Whole Cell Lysate
Western Blot with PSPH Monoclonal Antibody at dilution of 1:500. Lane A: MCF7 Whole Cell Lysate, Lane B: K562 Whole Cell Lysate, Lane C: HepG2 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.