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Antikörper

Recombinant MAX/MYC associated factor X Monoclonal Antibody

Produktgrößen
100 ul
£402,00
AN300263P-100UL
Über dieses Produkt
SKU:
AN300263P
Zusätzliche Namen:
bHLHd,MAX,bHLHd4,Class D Basic Helix-Loop-Helix Protein 4,Myc-Associated Factor X,Protein Max
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
0.2 um filtered solution in PBS with 10% Trehalose, pH7.0
translate.label.attr.clone:
B181
Klonalität:
Recombinant Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Mutations of this gene have been reported to be associated with hereditary pheochromocytoma. A pseudogene of this gene is located on the long arm of chromosome 4. Alternative splicing results in multiple transcript variants.
Host:
Rabbit
Immunogen:
Recombinant Human MAX / MYC associated factor X protein
Isotyp:
IgG
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Recombinant Antibodies
Flow cytometric analysis of Human MAX expression on Raji cells. The cells were stained with purified anti-Human MAX, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human MAX in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MAX Monoclonal Antibody (1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.
Western Blot with MAX / MYC associated factor X Monoclonal Antibody at dilution of 1:500. Lane A: Jurkat Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lysates/proteins at 30 ug per lane.