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Antikörper

Recombinant PARK7/DJ-1 Monoclonal Antibody

Produktgrößen
20 ul
£189,00
AN300257P-20UL
100 ul
£402,00
AN300257P-100UL
Über dieses Produkt
SKU:
AN300257P
Zusätzliche Namen:
GATD,PARK,DJ-1,DJ1,GATD2,HEL-S-67p,PARK7
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS
translate.label.attr.clone:
B175
Klonalität:
Recombinant Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 4. Two transcript variants encoding the same protein have been identified for this gene.
Host:
Rabbit
Immunogen:
Recombinant Human PARK7 / DJ-1 protein
Isotyp:
IgG
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Recombinant Antibodies
Flow cytometric analysis of Human PARK7 expression on HeLa cells. The cells were stained with purified anti-Human PARK7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human PARK7 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PARK7 Monoclonal Antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Immunoprecipitation analysis using 2 uL anti-PARK7 Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using PARK7 Monoclonal Antibody at a dilution of 1:1000. Lane A:0.5 mg Jurkat Whole Cell Lysate, Lane B:0.5 mg Hela Whole Cell Lysate, Lane C:0.5 mg 293T Whole Cell Lysate
Western Blot with PARK7 / DJ-1 Monoclonal Antibody at dilution of 1:1000. Lane A: Hela Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lysates/proteins at 30 ug per lane.