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Antikörper

Recombinant CRABP2 Monoclonal Antibody

Produktgrößen
100 ul
£402,00
AN300227P-100UL
Über dieses Produkt
SKU:
AN300227P
Zusätzliche Namen:
RBP,CRABP,CRABP2,CRABP-II,RBP6,cellular,Cellular retinoic acid binding protein 2,Cellular retinoic acid binding protein II,Cellular retinoic acid-binding protein 2,Cellular retinoic acid-binding protein II,CRABP II,CRABPII,RABP2,Rretinoic acid-binding protein,type II
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS
translate.label.attr.clone:
B145
Klonalität:
Recombinant Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Host:
Rabbit
Immunogen:
Recombinant Human CRABP2 protein
Isotyp:
IgG
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Recombinant Antibodies
Flow cytometric analysis of Human CRABP2 expression on MCF-7 cells. The cells were stained with purified anti-Human CRABP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human CRABP2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human CRABP2 Monoclonal Antibody (1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Immunoprecipitation analysis using 0.5 uL anti-CRABP2 Monoclonal Antibody and 60 ug of Immunomagnetic beads Protein G. Western blot was performed from the immunoprecipitate using CRABP2 Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate
Western Blot with CRABP2 Monoclonal Antibody at dilution of 1:500. Lane A: 293T Whole Cell Lysate, Lane B: MCF7 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.