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Antikörper

Recombinant Phospho-eIF2A Alpha (Ser51) Monoclonal Antibody

Produktgrößen
20 ul
£189,00
AN300145L-20UL
100 ul
£402,00
AN300145L-100UL
Über dieses Produkt
SKU:
AN300145L
Zusätzliche Namen:
EIF2S,EIF,EIF-2,EIF-2A,EIF-2alpha,EIF2,EIF2A,EIF2S1,EIF 2,EIF 2 alpha,EIF 2A,EIF 2alpha,EIF2 alpha,eIF-2-alpha,Eukaryotic translation initiation factor 2 subunit 1,Eukaryotic translation initiation factor 2 subunit 1 alpha,Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa,Eukaryotic translation initiation factor 2 subunit alpha,IF2A
Anwendung:
Western Blot
Buffer:
10 mM sodium HEPES, 150 mM NaCl, 100 ug/mL protein protectant, 50% glycerol, pH 7.5
translate.label.attr.clone:
A1259
Klonalität:
Recombinant Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2, MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2007]
Host:
Rabbit
Immunogen:
A synthetic phosphopeptide corresponding to residues around Ser51 of human Phospho-eIF2A Alpha.
Isotyp:
IgG
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Recombinant Antibodies
Western blot analysis of extracts from serum-starved Hela, untreated (line A), treated with Calyculin A (100nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-eIF2A Alpha (Ser51) Monoclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from serum-starved Hela, treated with Calyculin A (100 nM, 30 min), using Phospho-eIF2A Alpha (Ser51) Monoclonal Antibody and other brands' antibodies (company C) at dilution of 1:1000, 1:10000 and 1:200000.
Western blot analysis of extracts from serum-starved NIH-3T3, untreated(line A) or treated with CalyculinA( 100nM, 30min; +)(line B), using Phospho-eIF2A Alpha (Ser51) Monoclonal Antibody at 1:1000 dilution.