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Antikörper

Recombinant KYNU Monoclonal Antibody

Produktgrößen
100 ul
£402,00
AN300078P-100UL
Über dieses Produkt
SKU:
AN300078P
Zusätzliche Namen:
KYNU,KYNUU
Anwendung:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS
translate.label.attr.clone:
A1188
Klonalität:
Recombinant Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 0-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants.
Host:
Rabbit
Immunogen:
Recombinant Human KYNU Protein
Isotyp:
IgG
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Recombinant Antibodies
Immunofluorescence analysis of KYNU in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human KYNU Monoclonal Antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue).Positive staining was localized to Cytoplasm.
Immunoprecipitation analysis using 0.5 uL anti-KYNU-HIS Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using KYNU-HIS Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg A549 Whole Cell Lysate
Western Blot with KYNU Monoclonal Antibody at dilution of 1:500. Lane A: A549 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.