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Antikörper

Recombinant CD55/DAF Monoclonal Antibody

Produktgrößen
20 ul
£189,00
AN300017P-20UL
100 ul
£402,00
AN300017P-100UL
Über dieses Produkt
SKU:
AN300017P
Zusätzliche Namen:
CD55,CR,CROM,DAF,TC,Complement Decay-Accelerating factor,CD 55,CD55 antigen,CD55 Cromer blood group system,CD55 molecule,CD55 molecule (Cromer blood group),Cd55a,Complement decay accelerating factor,Cromer Blood Group antigen,Cromer blood group system,Cromer blood group system),Daf1,Daf-GPI,Dcay accelerating factor for complement (CD55,Decay accelarating factor 1,Decay accelerating factor (GPI-form),Decay Accelerating Factor for Complement,decay accelerating factor for complement (Cromer blood group),Decay accelerating factor GPI-form,Decay accelerating factor soluble-form,GPI-anchored,GPI-DAF,isoform CRA_a
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Buffer:
PBS
translate.label.attr.clone:
A1127
Klonalität:
Recombinant Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
CD55 is a 60-70 kD glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein also known as decay-accelerating factor (DAF). It is expressed on hematopoietic cells including erythrocytes and many non-hematopoietic cells. CD55 accelerates the dissociation of the components of the C3-convertases (namely C2a from C4b in the C4bC2a complex, a C3-convertase of the classical pathway, and factor Bb from the C3bBb complex, a C3-convertase of the alternative pathway) to protect cells from inappropriate damage caused by autologous complement. CD55 has been reported to reduce the efficiency of NK cell lysis and induce signal transduction in T cells. CD55 has also been shown to interact with CD97 and bind to Coxsackie and Echovirus.
Host:
Rabbit
Immunogen:
Recombinant Human CD55 protein
Isotyp:
IgG
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Recombinant Antibodies
Flow cytometric analysis of human CD55 expression on K562 cells. Cells were stained with purified anti-Human CD55, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence staining of Human CD55 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD55 Monoclonal Antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to plasma membrane.