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Antikörper

S100A5 Monoclonal Antibody

Produktgrößen
100 ul
£346,00
AN200207P-100UL
Über dieses Produkt
SKU:
AN200207P
Zusätzliche Namen:
S100A,S100A5,S100D
Anwendung:
Immunocytochemistry, Immunofluorescence
Buffer:
PBS
translate.label.attr.clone:
A1059
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain.
Host:
Mouse
Immunogen:
Recombinant Human S100A5 protein
Isotyp:
IgG1
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of Human S100A5 in A431 or Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human S100A5 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope, right panel, captured by fluorescence microscope) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.