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Antikörper

Cyclin A1/CCNA1 Monoclonal Antibody

Produktgrößen
100 ul
£346,00
AN200197P-100UL
Über dieses Produkt
SKU:
AN200197P
Zusätzliche Namen:
CCNA;cyclin-A;CCNA1;CT146;cyclin-A1;CCN-A1
Anwendung:
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Buffer:
0.2 um filtered solution in PBS
translate.label.attr.clone:
A1049
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene.
Host:
Mouse
Immunogen:
Recombinant Human Cyclin A1/CCNA1 protein
Isotyp:
IgG2b
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Immunofluorescence analysis of Human CCNA1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CCNA1 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.
Flow cytometric analysis of Human CCNA1 expression on HeLa cells. The cells were treated according to manufacturer's manual, stained with purified anti-Human CCNA1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.