Skip to content

Warenkorb

Der Warenkorb ist leer.

Gesamt (exkl. MwSt.) £0,00
Warenkorb überprüfen & bezahlen
Antikörper

KRT19 Monoclonal Antibody

Produktgrößen
100 ul
£346,00
AN200158P-100UL
Über dieses Produkt
SKU:
AN200158P
Zusätzliche Namen:
KRT19,Cytokeratin-19 (CK-19),Keratin-19 (K19)
Anwendung:
Flow Cytometry, Immunoprecipitation, Western Blot
Buffer:
PBS
translate.label.attr.clone:
A1010
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Weitere Details:
Cytokeratin 19 is part of a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented inHumanepithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40-68 kDa and isoelectric pH between 4.9 - 7.8. The individualHumancytokeratins are numbered 1 to 20. The various epithelia in theHumanbody usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. For example, cytokeratin 19 is not expressed in hepatocytes, therefore, it is useful in the identification of liver metastasis.
Host:
Mouse
Immunogen:
A synthetic peptide corresponding to the N-terminus of the human KRT19
Isotyp:
IgG3
Aufreinigung:
Protein A Purified
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Hersteller:
Elabscience
Typ:
Antibody: Monoclonal Antibody
Immunoprecipitation analysis using 4 uL anti-KRT19 Monoclonal Antibody and 15 ul of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using KRT19 Monoclonal Antibody at a dilution of 1:100. Lane A:0.5 mg HepG2 Whole Cell Lysate
Western Blot with KRT19 Monoclonal Antibody at dilution of 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: HCT116 Whole Cell Lysate, Lane C: A431 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.
Flow cytometric analysis of Human KRT19 expression on A431 cells. The cells were treated according to manufacturer's manual, stained with purified anti-Human KRT19, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Western Blot with KRT19 Monoclonal Antibody at dilution of 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: KRT19 konckout HepG2 Whole Cell Lysate, Lysates/proteins at 30 ug per lane.